human skin fibroblast cell line ws1 (ATCC)
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human skin fibroblast cell line ws1
Human Skin Fibroblast Cell Line Ws1, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 383 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ws1+human+skin+fibroblast+cell+line/WS1/pm41796259-219-1-11
Average 95 stars, based on 383 article reviews
Human Skin Fibroblast Cell Line Ws1, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 383 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ws1+human+skin+fibroblast+cell+line/WS1/pm41796259-219-1-11
Average 95 stars, based on 383 article reviews
human skin fibroblast cell line ws1 - by Bioz Stars,
2026-10
95/100 stars
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Purification:Article Title: EccDNA‐Driven VPS41 Amplification Alleviates Genotoxic Stress via Lysosomal KAI1 Degradation Article Snippet: The HaCaT (human keratinocyte) cell line was obtained from the German Cancer Research Center (Heidelberg, Germany) as previously reported ,[ ] and the Article Title: Antimicrobial Nitric Oxide-Releasing Electrospun Dressings for Wound Healing Applications Article Snippet: Gelatin (gel strength 300 g Bloom, Type A), poly(ε-caprolactone) (PCL) ( M n : 80 000 g·mol –1 ), genipin (≥98%), 2,2,2-trifluoroethanol (TFE), Luria-Bertani (LB) broth, agar, DMEM/F12, penicillin and streptomycin, biopore membrane filter roll, and hydrophilic PTFE were acquired from Merck (Gillingham, U.K.) and used as received.strength 300 g Bloom, Type A), poly(ε-caprolactone) (PCL) ( M n : 80 000 g·mol –1 ), genipin (≥98%), 2,2,2-trifluoroethanol (TFE), Luria-Bertani (LB) broth, agar, DMEM/F12, penicillin and streptomycin, biopore membrane filter roll, and hydrophilic PTFE were acquired from Merck (Gillingham, U.K.) and used as received. ... The human keratinocyte HaCaT cell line was kindly donated by Dr. Kevin Hamill (University of Liverpool, U.K.), and the Sequencing:Article Title: EccDNA‐Driven VPS41 Amplification Alleviates Genotoxic Stress via Lysosomal KAI1 Degradation Article Snippet: The HaCaT (human keratinocyte) cell line was obtained from the German Cancer Research Center (Heidelberg, Germany) as previously reported ,[ ] and the Article Title: Antimicrobial Nitric Oxide-Releasing Electrospun Dressings for Wound Healing Applications Article Snippet: Gelatin (gel strength 300 g Bloom, Type A), poly(ε-caprolactone) (PCL) ( M n : 80 000 g·mol –1 ), genipin (≥98%), 2,2,2-trifluoroethanol (TFE), Luria-Bertani (LB) broth, agar, DMEM/F12, penicillin and streptomycin, biopore membrane filter roll, and hydrophilic PTFE were acquired from Merck (Gillingham, U.K.) and used as received.strength 300 g Bloom, Type A), poly(ε-caprolactone) (PCL) ( M n : 80 000 g·mol –1 ), genipin (≥98%), 2,2,2-trifluoroethanol (TFE), Luria-Bertani (LB) broth, agar, DMEM/F12, penicillin and streptomycin, biopore membrane filter roll, and hydrophilic PTFE were acquired from Merck (Gillingham, U.K.) and used as received. ... The human keratinocyte HaCaT cell line was kindly donated by Dr. Kevin Hamill (University of Liverpool, U.K.), and the Nucleic Acid Electrophoresis:Article Title: EccDNA‐Driven VPS41 Amplification Alleviates Genotoxic Stress via Lysosomal KAI1 Degradation Article Snippet: The HaCaT (human keratinocyte) cell line was obtained from the German Cancer Research Center (Heidelberg, Germany) as previously reported ,[ ] and the Article Title: Antimicrobial Nitric Oxide-Releasing Electrospun Dressings for Wound Healing Applications Article Snippet: Gelatin (gel strength 300 g Bloom, Type A), poly(ε-caprolactone) (PCL) ( M n : 80 000 g·mol –1 ), genipin (≥98%), 2,2,2-trifluoroethanol (TFE), Luria-Bertani (LB) broth, agar, DMEM/F12, penicillin and streptomycin, biopore membrane filter roll, and hydrophilic PTFE were acquired from Merck (Gillingham, U.K.) and used as received.strength 300 g Bloom, Type A), poly(ε-caprolactone) (PCL) ( M n : 80 000 g·mol –1 ), genipin (≥98%), 2,2,2-trifluoroethanol (TFE), Luria-Bertani (LB) broth, agar, DMEM/F12, penicillin and streptomycin, biopore membrane filter roll, and hydrophilic PTFE were acquired from Merck (Gillingham, U.K.) and used as received. ... The human keratinocyte HaCaT cell line was kindly donated by Dr. Kevin Hamill (University of Liverpool, U.K.), and the Expressing:Article Title: EccDNA‐Driven VPS41 Amplification Alleviates Genotoxic Stress via Lysosomal KAI1 Degradation Article Snippet: The HaCaT (human keratinocyte) cell line was obtained from the German Cancer Research Center (Heidelberg, Germany) as previously reported ,[ ] and the Article Title: Antimicrobial Nitric Oxide-Releasing Electrospun Dressings for Wound Healing Applications Article Snippet: Gelatin (gel strength 300 g Bloom, Type A), poly(ε-caprolactone) (PCL) ( M n : 80 000 g·mol –1 ), genipin (≥98%), 2,2,2-trifluoroethanol (TFE), Luria-Bertani (LB) broth, agar, DMEM/F12, penicillin and streptomycin, biopore membrane filter roll, and hydrophilic PTFE were acquired from Merck (Gillingham, U.K.) and used as received.strength 300 g Bloom, Type A), poly(ε-caprolactone) (PCL) ( M n : 80 000 g·mol –1 ), genipin (≥98%), 2,2,2-trifluoroethanol (TFE), Luria-Bertani (LB) broth, agar, DMEM/F12, penicillin and streptomycin, biopore membrane filter roll, and hydrophilic PTFE were acquired from Merck (Gillingham, U.K.) and used as received. ... The human keratinocyte HaCaT cell line was kindly donated by Dr. Kevin Hamill (University of Liverpool, U.K.), and the Transfection:Article Title: EccDNA‐Driven VPS41 Amplification Alleviates Genotoxic Stress via Lysosomal KAI1 Degradation Article Snippet: The HaCaT (human keratinocyte) cell line was obtained from the German Cancer Research Center (Heidelberg, Germany) as previously reported ,[ ] and the Article Title: Antimicrobial Nitric Oxide-Releasing Electrospun Dressings for Wound Healing Applications Article Snippet: Gelatin (gel strength 300 g Bloom, Type A), poly(ε-caprolactone) (PCL) ( M n : 80 000 g·mol –1 ), genipin (≥98%), 2,2,2-trifluoroethanol (TFE), Luria-Bertani (LB) broth, agar, DMEM/F12, penicillin and streptomycin, biopore membrane filter roll, and hydrophilic PTFE were acquired from Merck (Gillingham, U.K.) and used as received.strength 300 g Bloom, Type A), poly(ε-caprolactone) (PCL) ( M n : 80 000 g·mol –1 ), genipin (≥98%), 2,2,2-trifluoroethanol (TFE), Luria-Bertani (LB) broth, agar, DMEM/F12, penicillin and streptomycin, biopore membrane filter roll, and hydrophilic PTFE were acquired from Merck (Gillingham, U.K.) and used as received. ... The human keratinocyte HaCaT cell line was kindly donated by Dr. Kevin Hamill (University of Liverpool, U.K.), and the Irradiation:Article Title: EccDNA‐Driven VPS41 Amplification Alleviates Genotoxic Stress via Lysosomal KAI1 Degradation Article Snippet: The HaCaT (human keratinocyte) cell line was obtained from the German Cancer Research Center (Heidelberg, Germany) as previously reported ,[ ] and the Article Title: Antimicrobial Nitric Oxide-Releasing Electrospun Dressings for Wound Healing Applications Article Snippet: Gelatin (gel strength 300 g Bloom, Type A), poly(ε-caprolactone) (PCL) ( M n : 80 000 g·mol –1 ), genipin (≥98%), 2,2,2-trifluoroethanol (TFE), Luria-Bertani (LB) broth, agar, DMEM/F12, penicillin and streptomycin, biopore membrane filter roll, and hydrophilic PTFE were acquired from Merck (Gillingham, U.K.) and used as received.strength 300 g Bloom, Type A), poly(ε-caprolactone) (PCL) ( M n : 80 000 g·mol –1 ), genipin (≥98%), 2,2,2-trifluoroethanol (TFE), Luria-Bertani (LB) broth, agar, DMEM/F12, penicillin and streptomycin, biopore membrane filter roll, and hydrophilic PTFE were acquired from Merck (Gillingham, U.K.) and used as received. ... The human keratinocyte HaCaT cell line was kindly donated by Dr. Kevin Hamill (University of Liverpool, U.K.), and the Injection:Article Title: EccDNA‐Driven VPS41 Amplification Alleviates Genotoxic Stress via Lysosomal KAI1 Degradation Article Snippet: The HaCaT (human keratinocyte) cell line was obtained from the German Cancer Research Center (Heidelberg, Germany) as previously reported ,[ ] and the Article Title: Antimicrobial Nitric Oxide-Releasing Electrospun Dressings for Wound Healing Applications Article Snippet: Gelatin (gel strength 300 g Bloom, Type A), poly(ε-caprolactone) (PCL) ( M n : 80 000 g·mol –1 ), genipin (≥98%), 2,2,2-trifluoroethanol (TFE), Luria-Bertani (LB) broth, agar, DMEM/F12, penicillin and streptomycin, biopore membrane filter roll, and hydrophilic PTFE were acquired from Merck (Gillingham, U.K.) and used as received.strength 300 g Bloom, Type A), poly(ε-caprolactone) (PCL) ( M n : 80 000 g·mol –1 ), genipin (≥98%), 2,2,2-trifluoroethanol (TFE), Luria-Bertani (LB) broth, agar, DMEM/F12, penicillin and streptomycin, biopore membrane filter roll, and hydrophilic PTFE were acquired from Merck (Gillingham, U.K.) and used as received. ... The human keratinocyte HaCaT cell line was kindly donated by Dr. Kevin Hamill (University of Liverpool, U.K.), and the Immunofluorescence:Article Title: EccDNA‐Driven VPS41 Amplification Alleviates Genotoxic Stress via Lysosomal KAI1 Degradation Article Snippet: The HaCaT (human keratinocyte) cell line was obtained from the German Cancer Research Center (Heidelberg, Germany) as previously reported ,[ ] and the Article Title: Antimicrobial Nitric Oxide-Releasing Electrospun Dressings for Wound Healing Applications Article Snippet: Gelatin (gel strength 300 g Bloom, Type A), poly(ε-caprolactone) (PCL) ( M n : 80 000 g·mol –1 ), genipin (≥98%), 2,2,2-trifluoroethanol (TFE), Luria-Bertani (LB) broth, agar, DMEM/F12, penicillin and streptomycin, biopore membrane filter roll, and hydrophilic PTFE were acquired from Merck (Gillingham, U.K.) and used as received.strength 300 g Bloom, Type A), poly(ε-caprolactone) (PCL) ( M n : 80 000 g·mol –1 ), genipin (≥98%), 2,2,2-trifluoroethanol (TFE), Luria-Bertani (LB) broth, agar, DMEM/F12, penicillin and streptomycin, biopore membrane filter roll, and hydrophilic PTFE were acquired from Merck (Gillingham, U.K.) and used as received. ... The human keratinocyte HaCaT cell line was kindly donated by Dr. Kevin Hamill (University of Liverpool, U.K.), and the MANN-WHITNEY:Article Title: EccDNA‐Driven VPS41 Amplification Alleviates Genotoxic Stress via Lysosomal KAI1 Degradation Article Snippet: The HaCaT (human keratinocyte) cell line was obtained from the German Cancer Research Center (Heidelberg, Germany) as previously reported ,[ ] and the Article Title: Antimicrobial Nitric Oxide-Releasing Electrospun Dressings for Wound Healing Applications Article Snippet: Gelatin (gel strength 300 g Bloom, Type A), poly(ε-caprolactone) (PCL) ( M n : 80 000 g·mol –1 ), genipin (≥98%), 2,2,2-trifluoroethanol (TFE), Luria-Bertani (LB) broth, agar, DMEM/F12, penicillin and streptomycin, biopore membrane filter roll, and hydrophilic PTFE were acquired from Merck (Gillingham, U.K.) and used as received.strength 300 g Bloom, Type A), poly(ε-caprolactone) (PCL) ( M n : 80 000 g·mol –1 ), genipin (≥98%), 2,2,2-trifluoroethanol (TFE), Luria-Bertani (LB) broth, agar, DMEM/F12, penicillin and streptomycin, biopore membrane filter roll, and hydrophilic PTFE were acquired from Merck (Gillingham, U.K.) and used as received. ... The human keratinocyte HaCaT cell line was kindly donated by Dr. Kevin Hamill (University of Liverpool, U.K.), and the High Throughput Screening Assay:Article Title: EccDNA‐Driven VPS41 Amplification Alleviates Genotoxic Stress via Lysosomal KAI1 Degradation Article Snippet: The HaCaT (human keratinocyte) cell line was obtained from the German Cancer Research Center (Heidelberg, Germany) as previously reported ,[ ] and the Article Title: Antimicrobial Nitric Oxide-Releasing Electrospun Dressings for Wound Healing Applications Article Snippet: Gelatin (gel strength 300 g Bloom, Type A), poly(ε-caprolactone) (PCL) ( M n : 80 000 g·mol –1 ), genipin (≥98%), 2,2,2-trifluoroethanol (TFE), Luria-Bertani (LB) broth, agar, DMEM/F12, penicillin and streptomycin, biopore membrane filter roll, and hydrophilic PTFE were acquired from Merck (Gillingham, U.K.) and used as received.strength 300 g Bloom, Type A), poly(ε-caprolactone) (PCL) ( M n : 80 000 g·mol –1 ), genipin (≥98%), 2,2,2-trifluoroethanol (TFE), Luria-Bertani (LB) broth, agar, DMEM/F12, penicillin and streptomycin, biopore membrane filter roll, and hydrophilic PTFE were acquired from Merck (Gillingham, U.K.) and used as received. ... The human keratinocyte HaCaT cell line was kindly donated by Dr. Kevin Hamill (University of Liverpool, U.K.), and the Biomarker Discovery:Article Title: EccDNA‐Driven VPS41 Amplification Alleviates Genotoxic Stress via Lysosomal KAI1 Degradation Article Snippet: The HaCaT (human keratinocyte) cell line was obtained from the German Cancer Research Center (Heidelberg, Germany) as previously reported ,[ ] and the Article Title: Antimicrobial Nitric Oxide-Releasing Electrospun Dressings for Wound Healing Applications Article Snippet: Gelatin (gel strength 300 g Bloom, Type A), poly(ε-caprolactone) (PCL) ( M n : 80 000 g·mol –1 ), genipin (≥98%), 2,2,2-trifluoroethanol (TFE), Luria-Bertani (LB) broth, agar, DMEM/F12, penicillin and streptomycin, biopore membrane filter roll, and hydrophilic PTFE were acquired from Merck (Gillingham, U.K.) and used as received.strength 300 g Bloom, Type A), poly(ε-caprolactone) (PCL) ( M n : 80 000 g·mol –1 ), genipin (≥98%), 2,2,2-trifluoroethanol (TFE), Luria-Bertani (LB) broth, agar, DMEM/F12, penicillin and streptomycin, biopore membrane filter roll, and hydrophilic PTFE were acquired from Merck (Gillingham, U.K.) and used as received. ... The human keratinocyte HaCaT cell line was kindly donated by Dr. Kevin Hamill (University of Liverpool, U.K.), and the Amplification:Article Title: EccDNA‐Driven VPS41 Amplification Alleviates Genotoxic Stress via Lysosomal KAI1 Degradation Article Snippet: The HaCaT (human keratinocyte) cell line was obtained from the German Cancer Research Center (Heidelberg, Germany) as previously reported ,[ ] and the Article Title: Antimicrobial Nitric Oxide-Releasing Electrospun Dressings for Wound Healing Applications Article Snippet: Gelatin (gel strength 300 g Bloom, Type A), poly(ε-caprolactone) (PCL) ( M n : 80 000 g·mol –1 ), genipin (≥98%), 2,2,2-trifluoroethanol (TFE), Luria-Bertani (LB) broth, agar, DMEM/F12, penicillin and streptomycin, biopore membrane filter roll, and hydrophilic PTFE were acquired from Merck (Gillingham, U.K.) and used as received.strength 300 g Bloom, Type A), poly(ε-caprolactone) (PCL) ( M n : 80 000 g·mol –1 ), genipin (≥98%), 2,2,2-trifluoroethanol (TFE), Luria-Bertani (LB) broth, agar, DMEM/F12, penicillin and streptomycin, biopore membrane filter roll, and hydrophilic PTFE were acquired from Merck (Gillingham, U.K.) and used as received. ... The human keratinocyte HaCaT cell line was kindly donated by Dr. Kevin Hamill (University of Liverpool, U.K.), and the Immunohistochemical staining:Article Title: EccDNA‐Driven VPS41 Amplification Alleviates Genotoxic Stress via Lysosomal KAI1 Degradation Article Snippet: The HaCaT (human keratinocyte) cell line was obtained from the German Cancer Research Center (Heidelberg, Germany) as previously reported ,[ ] and the Article Title: Antimicrobial Nitric Oxide-Releasing Electrospun Dressings for Wound Healing Applications Article Snippet: Gelatin (gel strength 300 g Bloom, Type A), poly(ε-caprolactone) (PCL) ( M n : 80 000 g·mol –1 ), genipin (≥98%), 2,2,2-trifluoroethanol (TFE), Luria-Bertani (LB) broth, agar, DMEM/F12, penicillin and streptomycin, biopore membrane filter roll, and hydrophilic PTFE were acquired from Merck (Gillingham, U.K.) and used as received.strength 300 g Bloom, Type A), poly(ε-caprolactone) (PCL) ( M n : 80 000 g·mol –1 ), genipin (≥98%), 2,2,2-trifluoroethanol (TFE), Luria-Bertani (LB) broth, agar, DMEM/F12, penicillin and streptomycin, biopore membrane filter roll, and hydrophilic PTFE were acquired from Merck (Gillingham, U.K.) and used as received. ... The human keratinocyte HaCaT cell line was kindly donated by Dr. Kevin Hamill (University of Liverpool, U.K.), and the Derivative Assay:Article Title: EccDNA‐Driven VPS41 Amplification Alleviates Genotoxic Stress via Lysosomal KAI1 Degradation Article Snippet: The HaCaT (human keratinocyte) cell line was obtained from the German Cancer Research Center (Heidelberg, Germany) as previously reported ,[ ] and the Article Title: Antimicrobial Nitric Oxide-Releasing Electrospun Dressings for Wound Healing Applications Article Snippet: Gelatin (gel strength 300 g Bloom, Type A), poly(ε-caprolactone) (PCL) ( M n : 80 000 g·mol –1 ), genipin (≥98%), 2,2,2-trifluoroethanol (TFE), Luria-Bertani (LB) broth, agar, DMEM/F12, penicillin and streptomycin, biopore membrane filter roll, and hydrophilic PTFE were acquired from Merck (Gillingham, U.K.) and used as received.strength 300 g Bloom, Type A), poly(ε-caprolactone) (PCL) ( M n : 80 000 g·mol –1 ), genipin (≥98%), 2,2,2-trifluoroethanol (TFE), Luria-Bertani (LB) broth, agar, DMEM/F12, penicillin and streptomycin, biopore membrane filter roll, and hydrophilic PTFE were acquired from Merck (Gillingham, U.K.) and used as received. ... The human keratinocyte HaCaT cell line was kindly donated by Dr. Kevin Hamill (University of Liverpool, U.K.), and the Gene Expression:Article Title: EccDNA‐Driven VPS41 Amplification Alleviates Genotoxic Stress via Lysosomal KAI1 Degradation Article Snippet: The HaCaT (human keratinocyte) cell line was obtained from the German Cancer Research Center (Heidelberg, Germany) as previously reported ,[ ] and the Article Title: Antimicrobial Nitric Oxide-Releasing Electrospun Dressings for Wound Healing Applications Article Snippet: Gelatin (gel strength 300 g Bloom, Type A), poly(ε-caprolactone) (PCL) ( M n : 80 000 g·mol –1 ), genipin (≥98%), 2,2,2-trifluoroethanol (TFE), Luria-Bertani (LB) broth, agar, DMEM/F12, penicillin and streptomycin, biopore membrane filter roll, and hydrophilic PTFE were acquired from Merck (Gillingham, U.K.) and used as received.strength 300 g Bloom, Type A), poly(ε-caprolactone) (PCL) ( M n : 80 000 g·mol –1 ), genipin (≥98%), 2,2,2-trifluoroethanol (TFE), Luria-Bertani (LB) broth, agar, DMEM/F12, penicillin and streptomycin, biopore membrane filter roll, and hydrophilic PTFE were acquired from Merck (Gillingham, U.K.) and used as received. ... The human keratinocyte HaCaT cell line was kindly donated by Dr. Kevin Hamill (University of Liverpool, U.K.), and the Plasmid Preparation:Article Title: EccDNA‐Driven VPS41 Amplification Alleviates Genotoxic Stress via Lysosomal KAI1 Degradation Article Snippet: The HaCaT (human keratinocyte) cell line was obtained from the German Cancer Research Center (Heidelberg, Germany) as previously reported ,[ ] and the Article Title: Antimicrobial Nitric Oxide-Releasing Electrospun Dressings for Wound Healing Applications Article Snippet: Gelatin (gel strength 300 g Bloom, Type A), poly(ε-caprolactone) (PCL) ( M n : 80 000 g·mol –1 ), genipin (≥98%), 2,2,2-trifluoroethanol (TFE), Luria-Bertani (LB) broth, agar, DMEM/F12, penicillin and streptomycin, biopore membrane filter roll, and hydrophilic PTFE were acquired from Merck (Gillingham, U.K.) and used as received.strength 300 g Bloom, Type A), poly(ε-caprolactone) (PCL) ( M n : 80 000 g·mol –1 ), genipin (≥98%), 2,2,2-trifluoroethanol (TFE), Luria-Bertani (LB) broth, agar, DMEM/F12, penicillin and streptomycin, biopore membrane filter roll, and hydrophilic PTFE were acquired from Merck (Gillingham, U.K.) and used as received. ... The human keratinocyte HaCaT cell line was kindly donated by Dr. Kevin Hamill (University of Liverpool, U.K.), and the Western Blot:Article Title: EccDNA‐Driven VPS41 Amplification Alleviates Genotoxic Stress via Lysosomal KAI1 Degradation Article Snippet: The HaCaT (human keratinocyte) cell line was obtained from the German Cancer Research Center (Heidelberg, Germany) as previously reported ,[ ] and the Article Title: Antimicrobial Nitric Oxide-Releasing Electrospun Dressings for Wound Healing Applications Article Snippet: Gelatin (gel strength 300 g Bloom, Type A), poly(ε-caprolactone) (PCL) ( M n : 80 000 g·mol –1 ), genipin (≥98%), 2,2,2-trifluoroethanol (TFE), Luria-Bertani (LB) broth, agar, DMEM/F12, penicillin and streptomycin, biopore membrane filter roll, and hydrophilic PTFE were acquired from Merck (Gillingham, U.K.) and used as received.strength 300 g Bloom, Type A), poly(ε-caprolactone) (PCL) ( M n : 80 000 g·mol –1 ), genipin (≥98%), 2,2,2-trifluoroethanol (TFE), Luria-Bertani (LB) broth, agar, DMEM/F12, penicillin and streptomycin, biopore membrane filter roll, and hydrophilic PTFE were acquired from Merck (Gillingham, U.K.) and used as received. ... The human keratinocyte HaCaT cell line was kindly donated by Dr. Kevin Hamill (University of Liverpool, U.K.), and the Over Expression:Article Title: EccDNA‐Driven VPS41 Amplification Alleviates Genotoxic Stress via Lysosomal KAI1 Degradation Article Snippet: The HaCaT (human keratinocyte) cell line was obtained from the German Cancer Research Center (Heidelberg, Germany) as previously reported ,[ ] and the Article Title: Antimicrobial Nitric Oxide-Releasing Electrospun Dressings for Wound Healing Applications Article Snippet: Gelatin (gel strength 300 g Bloom, Type A), poly(ε-caprolactone) (PCL) ( M n : 80 000 g·mol –1 ), genipin (≥98%), 2,2,2-trifluoroethanol (TFE), Luria-Bertani (LB) broth, agar, DMEM/F12, penicillin and streptomycin, biopore membrane filter roll, and hydrophilic PTFE were acquired from Merck (Gillingham, U.K.) and used as received.strength 300 g Bloom, Type A), poly(ε-caprolactone) (PCL) ( M n : 80 000 g·mol –1 ), genipin (≥98%), 2,2,2-trifluoroethanol (TFE), Luria-Bertani (LB) broth, agar, DMEM/F12, penicillin and streptomycin, biopore membrane filter roll, and hydrophilic PTFE were acquired from Merck (Gillingham, U.K.) and used as received. ... The human keratinocyte HaCaT cell line was kindly donated by Dr. Kevin Hamill (University of Liverpool, U.K.), and the Knockdown:Article Title: EccDNA‐Driven VPS41 Amplification Alleviates Genotoxic Stress via Lysosomal KAI1 Degradation Article Snippet: The HaCaT (human keratinocyte) cell line was obtained from the German Cancer Research Center (Heidelberg, Germany) as previously reported ,[ ] and the Article Title: Antimicrobial Nitric Oxide-Releasing Electrospun Dressings for Wound Healing Applications Article Snippet: Gelatin (gel strength 300 g Bloom, Type A), poly(ε-caprolactone) (PCL) ( M n : 80 000 g·mol –1 ), genipin (≥98%), 2,2,2-trifluoroethanol (TFE), Luria-Bertani (LB) broth, agar, DMEM/F12, penicillin and streptomycin, biopore membrane filter roll, and hydrophilic PTFE were acquired from Merck (Gillingham, U.K.) and used as received.strength 300 g Bloom, Type A), poly(ε-caprolactone) (PCL) ( M n : 80 000 g·mol –1 ), genipin (≥98%), 2,2,2-trifluoroethanol (TFE), Luria-Bertani (LB) broth, agar, DMEM/F12, penicillin and streptomycin, biopore membrane filter roll, and hydrophilic PTFE were acquired from Merck (Gillingham, U.K.) and used as received. ... The human keratinocyte HaCaT cell line was kindly donated by Dr. Kevin Hamill (University of Liverpool, U.K.), and the Infection:Article Title: EccDNA‐Driven VPS41 Amplification Alleviates Genotoxic Stress via Lysosomal KAI1 Degradation Article Snippet: The HaCaT (human keratinocyte) cell line was obtained from the German Cancer Research Center (Heidelberg, Germany) as previously reported ,[ ] and the Article Title: Antimicrobial Nitric Oxide-Releasing Electrospun Dressings for Wound Healing Applications Article Snippet: Gelatin (gel strength 300 g Bloom, Type A), poly(ε-caprolactone) (PCL) ( M n : 80 000 g·mol –1 ), genipin (≥98%), 2,2,2-trifluoroethanol (TFE), Luria-Bertani (LB) broth, agar, DMEM/F12, penicillin and streptomycin, biopore membrane filter roll, and hydrophilic PTFE were acquired from Merck (Gillingham, U.K.) and used as received.strength 300 g Bloom, Type A), poly(ε-caprolactone) (PCL) ( M n : 80 000 g·mol –1 ), genipin (≥98%), 2,2,2-trifluoroethanol (TFE), Luria-Bertani (LB) broth, agar, DMEM/F12, penicillin and streptomycin, biopore membrane filter roll, and hydrophilic PTFE were acquired from Merck (Gillingham, U.K.) and used as received. ... The human keratinocyte HaCaT cell line was kindly donated by Dr. Kevin Hamill (University of Liverpool, U.K.), and the Staining:Article Title: EccDNA‐Driven VPS41 Amplification Alleviates Genotoxic Stress via Lysosomal KAI1 Degradation Article Snippet: The HaCaT (human keratinocyte) cell line was obtained from the German Cancer Research Center (Heidelberg, Germany) as previously reported ,[ ] and the Article Title: Antimicrobial Nitric Oxide-Releasing Electrospun Dressings for Wound Healing Applications Article Snippet: Gelatin (gel strength 300 g Bloom, Type A), poly(ε-caprolactone) (PCL) ( M n : 80 000 g·mol –1 ), genipin (≥98%), 2,2,2-trifluoroethanol (TFE), Luria-Bertani (LB) broth, agar, DMEM/F12, penicillin and streptomycin, biopore membrane filter roll, and hydrophilic PTFE were acquired from Merck (Gillingham, U.K.) and used as received.strength 300 g Bloom, Type A), poly(ε-caprolactone) (PCL) ( M n : 80 000 g·mol –1 ), genipin (≥98%), 2,2,2-trifluoroethanol (TFE), Luria-Bertani (LB) broth, agar, DMEM/F12, penicillin and streptomycin, biopore membrane filter roll, and hydrophilic PTFE were acquired from Merck (Gillingham, U.K.) and used as received. ... The human keratinocyte HaCaT cell line was kindly donated by Dr. Kevin Hamill (University of Liverpool, U.K.), and the Mass Spectrometry:Article Title: EccDNA‐Driven VPS41 Amplification Alleviates Genotoxic Stress via Lysosomal KAI1 Degradation Article Snippet: The HaCaT (human keratinocyte) cell line was obtained from the German Cancer Research Center (Heidelberg, Germany) as previously reported ,[ ] and the Article Title: Antimicrobial Nitric Oxide-Releasing Electrospun Dressings for Wound Healing Applications Article Snippet: Gelatin (gel strength 300 g Bloom, Type A), poly(ε-caprolactone) (PCL) ( M n : 80 000 g·mol –1 ), genipin (≥98%), 2,2,2-trifluoroethanol (TFE), Luria-Bertani (LB) broth, agar, DMEM/F12, penicillin and streptomycin, biopore membrane filter roll, and hydrophilic PTFE were acquired from Merck (Gillingham, U.K.) and used as received.strength 300 g Bloom, Type A), poly(ε-caprolactone) (PCL) ( M n : 80 000 g·mol –1 ), genipin (≥98%), 2,2,2-trifluoroethanol (TFE), Luria-Bertani (LB) broth, agar, DMEM/F12, penicillin and streptomycin, biopore membrane filter roll, and hydrophilic PTFE were acquired from Merck (Gillingham, U.K.) and used as received. ... The human keratinocyte HaCaT cell line was kindly donated by Dr. Kevin Hamill (University of Liverpool, U.K.), and the Electron Microscopy:Article Title: EccDNA‐Driven VPS41 Amplification Alleviates Genotoxic Stress via Lysosomal KAI1 Degradation Article Snippet: The HaCaT (human keratinocyte) cell line was obtained from the German Cancer Research Center (Heidelberg, Germany) as previously reported ,[ ] and the Article Title: Antimicrobial Nitric Oxide-Releasing Electrospun Dressings for Wound Healing Applications Article Snippet: Gelatin (gel strength 300 g Bloom, Type A), poly(ε-caprolactone) (PCL) ( M n : 80 000 g·mol –1 ), genipin (≥98%), 2,2,2-trifluoroethanol (TFE), Luria-Bertani (LB) broth, agar, DMEM/F12, penicillin and streptomycin, biopore membrane filter roll, and hydrophilic PTFE were acquired from Merck (Gillingham, U.K.) and used as received.strength 300 g Bloom, Type A), poly(ε-caprolactone) (PCL) ( M n : 80 000 g·mol –1 ), genipin (≥98%), 2,2,2-trifluoroethanol (TFE), Luria-Bertani (LB) broth, agar, DMEM/F12, penicillin and streptomycin, biopore membrane filter roll, and hydrophilic PTFE were acquired from Merck (Gillingham, U.K.) and used as received. ... The human keratinocyte HaCaT cell line was kindly donated by Dr. Kevin Hamill (University of Liverpool, U.K.), and the Confocal Microscopy:Article Title: EccDNA‐Driven VPS41 Amplification Alleviates Genotoxic Stress via Lysosomal KAI1 Degradation Article Snippet: The HaCaT (human keratinocyte) cell line was obtained from the German Cancer Research Center (Heidelberg, Germany) as previously reported ,[ ] and the Article Title: Antimicrobial Nitric Oxide-Releasing Electrospun Dressings for Wound Healing Applications Article Snippet: Gelatin (gel strength 300 g Bloom, Type A), poly(ε-caprolactone) (PCL) ( M n : 80 000 g·mol –1 ), genipin (≥98%), 2,2,2-trifluoroethanol (TFE), Luria-Bertani (LB) broth, agar, DMEM/F12, penicillin and streptomycin, biopore membrane filter roll, and hydrophilic PTFE were acquired from Merck (Gillingham, U.K.) and used as received.strength 300 g Bloom, Type A), poly(ε-caprolactone) (PCL) ( M n : 80 000 g·mol –1 ), genipin (≥98%), 2,2,2-trifluoroethanol (TFE), Luria-Bertani (LB) broth, agar, DMEM/F12, penicillin and streptomycin, biopore membrane filter roll, and hydrophilic PTFE were acquired from Merck (Gillingham, U.K.) and used as received. ... The human keratinocyte HaCaT cell line was kindly donated by Dr. Kevin Hamill (University of Liverpool, U.K.), and the Immunoprecipitation:Article Title: EccDNA‐Driven VPS41 Amplification Alleviates Genotoxic Stress via Lysosomal KAI1 Degradation Article Snippet: The HaCaT (human keratinocyte) cell line was obtained from the German Cancer Research Center (Heidelberg, Germany) as previously reported ,[ ] and the Article Title: Antimicrobial Nitric Oxide-Releasing Electrospun Dressings for Wound Healing Applications Article Snippet: Gelatin (gel strength 300 g Bloom, Type A), poly(ε-caprolactone) (PCL) ( M n : 80 000 g·mol –1 ), genipin (≥98%), 2,2,2-trifluoroethanol (TFE), Luria-Bertani (LB) broth, agar, DMEM/F12, penicillin and streptomycin, biopore membrane filter roll, and hydrophilic PTFE were acquired from Merck (Gillingham, U.K.) and used as received.strength 300 g Bloom, Type A), poly(ε-caprolactone) (PCL) ( M n : 80 000 g·mol –1 ), genipin (≥98%), 2,2,2-trifluoroethanol (TFE), Luria-Bertani (LB) broth, agar, DMEM/F12, penicillin and streptomycin, biopore membrane filter roll, and hydrophilic PTFE were acquired from Merck (Gillingham, U.K.) and used as received. ... The human keratinocyte HaCaT cell line was kindly donated by Dr. Kevin Hamill (University of Liverpool, U.K.), and the Two Tailed Test:Article Title: EccDNA‐Driven VPS41 Amplification Alleviates Genotoxic Stress via Lysosomal KAI1 Degradation Article Snippet: The HaCaT (human keratinocyte) cell line was obtained from the German Cancer Research Center (Heidelberg, Germany) as previously reported ,[ ] and the Article Title: Antimicrobial Nitric Oxide-Releasing Electrospun Dressings for Wound Healing Applications Article Snippet: Gelatin (gel strength 300 g Bloom, Type A), poly(ε-caprolactone) (PCL) ( M n : 80 000 g·mol –1 ), genipin (≥98%), 2,2,2-trifluoroethanol (TFE), Luria-Bertani (LB) broth, agar, DMEM/F12, penicillin and streptomycin, biopore membrane filter roll, and hydrophilic PTFE were acquired from Merck (Gillingham, U.K.) and used as received.strength 300 g Bloom, Type A), poly(ε-caprolactone) (PCL) ( M n : 80 000 g·mol –1 ), genipin (≥98%), 2,2,2-trifluoroethanol (TFE), Luria-Bertani (LB) broth, agar, DMEM/F12, penicillin and streptomycin, biopore membrane filter roll, and hydrophilic PTFE were acquired from Merck (Gillingham, U.K.) and used as received. ... The human keratinocyte HaCaT cell line was kindly donated by Dr. Kevin Hamill (University of Liverpool, U.K.), and the Cotransfection:Article Title: EccDNA‐Driven VPS41 Amplification Alleviates Genotoxic Stress via Lysosomal KAI1 Degradation Article Snippet: The HaCaT (human keratinocyte) cell line was obtained from the German Cancer Research Center (Heidelberg, Germany) as previously reported ,[ ] and the Article Title: Antimicrobial Nitric Oxide-Releasing Electrospun Dressings for Wound Healing Applications Article Snippet: Gelatin (gel strength 300 g Bloom, Type A), poly(ε-caprolactone) (PCL) ( M n : 80 000 g·mol –1 ), genipin (≥98%), 2,2,2-trifluoroethanol (TFE), Luria-Bertani (LB) broth, agar, DMEM/F12, penicillin and streptomycin, biopore membrane filter roll, and hydrophilic PTFE were acquired from Merck (Gillingham, U.K.) and used as received.strength 300 g Bloom, Type A), poly(ε-caprolactone) (PCL) ( M n : 80 000 g·mol –1 ), genipin (≥98%), 2,2,2-trifluoroethanol (TFE), Luria-Bertani (LB) broth, agar, DMEM/F12, penicillin and streptomycin, biopore membrane filter roll, and hydrophilic PTFE were acquired from Merck (Gillingham, U.K.) and used as received. ... The human keratinocyte HaCaT cell line was kindly donated by Dr. Kevin Hamill (University of Liverpool, U.K.), and the Peptide Microarray:Article Title: EccDNA‐Driven VPS41 Amplification Alleviates Genotoxic Stress via Lysosomal KAI1 Degradation Article Snippet: The HaCaT (human keratinocyte) cell line was obtained from the German Cancer Research Center (Heidelberg, Germany) as previously reported ,[ ] and the Article Title: Antimicrobial Nitric Oxide-Releasing Electrospun Dressings for Wound Healing Applications Article Snippet: Gelatin (gel strength 300 g Bloom, Type A), poly(ε-caprolactone) (PCL) ( M n : 80 000 g·mol –1 ), genipin (≥98%), 2,2,2-trifluoroethanol (TFE), Luria-Bertani (LB) broth, agar, DMEM/F12, penicillin and streptomycin, biopore membrane filter roll, and hydrophilic PTFE were acquired from Merck (Gillingham, U.K.) and used as received.strength 300 g Bloom, Type A), poly(ε-caprolactone) (PCL) ( M n : 80 000 g·mol –1 ), genipin (≥98%), 2,2,2-trifluoroethanol (TFE), Luria-Bertani (LB) broth, agar, DMEM/F12, penicillin and streptomycin, biopore membrane filter roll, and hydrophilic PTFE were acquired from Merck (Gillingham, U.K.) and used as received. ... The human keratinocyte HaCaT cell line was kindly donated by Dr. Kevin Hamill (University of Liverpool, U.K.), and the Imaging:Article Title: EccDNA‐Driven VPS41 Amplification Alleviates Genotoxic Stress via Lysosomal KAI1 Degradation Article Snippet: The HaCaT (human keratinocyte) cell line was obtained from the German Cancer Research Center (Heidelberg, Germany) as previously reported ,[ ] and the Article Title: Antimicrobial Nitric Oxide-Releasing Electrospun Dressings for Wound Healing Applications Article Snippet: Gelatin (gel strength 300 g Bloom, Type A), poly(ε-caprolactone) (PCL) ( M n : 80 000 g·mol –1 ), genipin (≥98%), 2,2,2-trifluoroethanol (TFE), Luria-Bertani (LB) broth, agar, DMEM/F12, penicillin and streptomycin, biopore membrane filter roll, and hydrophilic PTFE were acquired from Merck (Gillingham, U.K.) and used as received.strength 300 g Bloom, Type A), poly(ε-caprolactone) (PCL) ( M n : 80 000 g·mol –1 ), genipin (≥98%), 2,2,2-trifluoroethanol (TFE), Luria-Bertani (LB) broth, agar, DMEM/F12, penicillin and streptomycin, biopore membrane filter roll, and hydrophilic PTFE were acquired from Merck (Gillingham, U.K.) and used as received. ... The human keratinocyte HaCaT cell line was kindly donated by Dr. Kevin Hamill (University of Liverpool, U.K.), and the Protein Binding:Article Title: EccDNA‐Driven VPS41 Amplification Alleviates Genotoxic Stress via Lysosomal KAI1 Degradation Article Snippet: The HaCaT (human keratinocyte) cell line was obtained from the German Cancer Research Center (Heidelberg, Germany) as previously reported ,[ ] and the Article Title: Antimicrobial Nitric Oxide-Releasing Electrospun Dressings for Wound Healing Applications Article Snippet: Gelatin (gel strength 300 g Bloom, Type A), poly(ε-caprolactone) (PCL) ( M n : 80 000 g·mol –1 ), genipin (≥98%), 2,2,2-trifluoroethanol (TFE), Luria-Bertani (LB) broth, agar, DMEM/F12, penicillin and streptomycin, biopore membrane filter roll, and hydrophilic PTFE were acquired from Merck (Gillingham, U.K.) and used as received.strength 300 g Bloom, Type A), poly(ε-caprolactone) (PCL) ( M n : 80 000 g·mol –1 ), genipin (≥98%), 2,2,2-trifluoroethanol (TFE), Luria-Bertani (LB) broth, agar, DMEM/F12, penicillin and streptomycin, biopore membrane filter roll, and hydrophilic PTFE were acquired from Merck (Gillingham, U.K.) and used as received. ... The human keratinocyte HaCaT cell line was kindly donated by Dr. Kevin Hamill (University of Liverpool, U.K.), and the Binding Assay:Article Title: EccDNA‐Driven VPS41 Amplification Alleviates Genotoxic Stress via Lysosomal KAI1 Degradation Article Snippet: The HaCaT (human keratinocyte) cell line was obtained from the German Cancer Research Center (Heidelberg, Germany) as previously reported ,[ ] and the Article Title: Antimicrobial Nitric Oxide-Releasing Electrospun Dressings for Wound Healing Applications Article Snippet: Gelatin (gel strength 300 g Bloom, Type A), poly(ε-caprolactone) (PCL) ( M n : 80 000 g·mol –1 ), genipin (≥98%), 2,2,2-trifluoroethanol (TFE), Luria-Bertani (LB) broth, agar, DMEM/F12, penicillin and streptomycin, biopore membrane filter roll, and hydrophilic PTFE were acquired from Merck (Gillingham, U.K.) and used as received.strength 300 g Bloom, Type A), poly(ε-caprolactone) (PCL) ( M n : 80 000 g·mol –1 ), genipin (≥98%), 2,2,2-trifluoroethanol (TFE), Luria-Bertani (LB) broth, agar, DMEM/F12, penicillin and streptomycin, biopore membrane filter roll, and hydrophilic PTFE were acquired from Merck (Gillingham, U.K.) and used as received. ... The human keratinocyte HaCaT cell line was kindly donated by Dr. Kevin Hamill (University of Liverpool, U.K.), and the |
![VPS41 negatively regulates KAI1 expression through the lysosomal pathway to confer resistance to apoptosis. A) Flowchart for screening VPS41 interaction proteins via differential protein analysis and mass spectrometry after VPS41 upregulation post‐irradiation. B) Volcano plot showing proteomic analysis (VPS41 vs Vector). C) Electron microscopy analysis reveals inhibited apoptosis progression in cells with upregulated VPS41 after irradiation (scale bar: 5 µm). D) Intersection of differential proteins identified four candidates: ISG15, KAI1, IFT20, and ATPAF1. E) Co‐localization of VPS41‐EGFP and KAI1‐BFP plasmids in WS1 and HaCaT cells assessed by confocal microscopy (scale bar: 20 µm). F) Immunoprecipitation confirms VPS41 binds KAI1. G) PNGase F treatment has minimal effect on VPS41‐KAI1 interaction. H) Western Blot shows upregulation of VPS41 decreases KAI1 expression, suppressing apoptosis, while VPS41 downregulation increases KAI1 expression and enhances apoptosis. I) VPS41 and KAI1 expression changes in HaCaT cells treated with CQ (20µ m ) or MG‐132 (20 µ m ) combined with X‐ray (10 Gy). J) Analysis of KAI1 decay rate after CHX (300 µ m ) treatment and X‐ray (10 Gy) in HaCaT cells. K) Effect of VPS41 knockdown and eccDNA transfection on apoptosis rates in irradiated cells with or without KAI1 overexpression. L) Apoptosis testing shows KAI1 reverses VPS41‐mediated radiation resistance. M) LDH measurement assesses the role of KAI1 in reversing VPS41‐mediated radiation resistance. P values were calculated using different statistical methods based on data type: unpaired two‐tailed t test for differential protein analysis and one‐way ANOVA followed by Bonferroni's post hoc test for multi‐group comparisons. Statistically significant differences are denoted as follows: * p < 0.05, ** p < 0.01. Data are presented as mean ± SD ( n = 3) unless otherwise specified. [Correction added on 28 April 2025, after first online publication: figure 5 is updated in this version].](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_4964/pmc12224964/pmc12224964__ADVS-12-2501934-g007.jpg)
